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Bioscience, Biotechnology, and Biochemistry Volume 64, 2000 - Issue 3
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Original Articles

Cloning of a Full-length cDNA Encoding ent-Kaurene Synthase from Gibberella fujikuroi: Functional Analysis of a Bifunctional Diterpene Cyclase

Tomonobu TOYOMASUDepartment of Bioresource Engineering, Yamagata University
,
Hiroshi KAWAIDEFrontier Research Program, The Institute of Physical and Chemical Research (RIKEN)
,
Atsuko ISHIZAKIDepartment of Bioresource Engineering, Yamagata University
,
Shoko SHINODADepartment of Bioresource Engineering, Yamagata University
,
Minoru OTSUKADepartment of Bioresource Engineering, Yamagata University
,
Wataru MITSUHASHIDepartment of Bioresource Engineering, Yamagata University
&
Takeshi SASSADepartment of Bioresource Engineering, Yamagata University
 show all
Pages 660-664 | Received 12 Oct 1999, Accepted 15 Nov 1999, Published online: 22 May 2014
 
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Abstract

We report here the nucleotide sequence of a full-length cDNA encoding ent-kaurene synthase that was isolated by a reverse-transcription polymerase chain reaction from Gibberella fujikuroi (Gcps/ks). This cDNA encodes 952 amino acid residues with a relative molecular mass of 107 kDa. The sequence similarity between Gcps/ks and ent-kaurene synthase of the gibberellin A1-producing fungus, Phaeosphaeria sp. L487, is very high, suggesting that Gcps/ks is also a bifunctional diterpene cyclase. Its recombinant protein expressed in Escherichia coli converted geranylgeranyl diphosphate to copalyl diphosphate and ent-kaurene.

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Related Research Data

Ent-kaurene synthase from the fungus Phaeosphaeria sp. L487. cDNA isolation, characterization, and bacterial expression of a bifunctional diterpene cyclase in fungal gibberellin biosynthesis.
Source: American Society for Biochemistry & Molecular Biology (ASBMB)
Gibberellin biosynthesis in bacteria: Separate ent-copalyl diphosphate and ent-kaurene synthases in Bradyrhizobium japonicum
Source: Wiley
Phytochrome Regulates Gibberellin Biosynthesis during Germination of Photoblastic Lettuce Seeds
Source: American Society of Plant Biologists (ASPB)
Gibberellin biosynthesis in Gibberella fujikuroi: cloning and characterization of the copalyl diphosphate synthase gene.
Source: Springer Science and Business Media LLC
Plant-like biosynthesis of gibberellin A1 in the fungus Phaeosphaeria sp. L487
Source: Elsevier BV
The biosynthesis of a C19-gibberellin from mevalonic acid in a cell-free system from a higher plant.
Source: Springer Science and Business Media LLC
Phytochemical Analysis, Biochemical and Mineral Composition and GC-MS Profiling of Methanolic Extract of Chinese Arrowhead Sagittaria trifolia L. from Northeast China.
Source: MDPI AG
Functional Analysis of the Two Interacting Cyclase Domains in ent-Kaurene Synthase from the FungusPhaeosphaeria sp. L487 and a Comparison with Cyclases from Higher Plants
Source: Elsevier BV
Accumulation of Gibberellin A1 and the Metabolism of Gibberellin A9 to Gibberellin A1 in a Phaeosphaeria sp. L487 Culture
Source: Oxford University Press (OUP)
Characterization of the final two genes of the gibberellin biosynthesis gene cluster of Gibberella fujikuroi: des and P450-3 encode GA4 desaturase and the 13-hydroxylase, respectively.
Source: American Society for Biochemistry & Molecular Biology (ASBMB)
Terpene synthase family
Source: Wiley

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