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Original Articles

Storage-dependent Degradation of 57-kDa Protein in Royal Jelly: a Possible Marker for Freshness

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Pages 277-284 | Received 10 Jul 2000, Accepted 18 Oct 2000, Published online: 22 May 2014
 

Abstract

In order to find a maker for freshness of royal jelly (RJ), the composition change of RJ during storage was investigated. The contents of 10-hydroxy-2-decenoic acid, a bioactive component of RJ, and several vitamins did not change during storage at 40°C for 7 days. However, a specific protein, designated royal jelly protein-1 (RJP-1), was gradually degraded during storage under various conditions (from 4°C to 50°C for up to 7 days). The specific degradation of RJP-1 was proportional to storage temperature and storage period. RJP-1 was purified to homogeneity and characterized as a monomeric glycoprotein with a molecular mass of 57 kDa. These results suggest that 57-kDa protein in RJ can be used as a marker for freshness of RJ, reflecting the conditions under which RJ has been stored.

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