Isolation and Primary Structural Analysis of Two Conjugated Polyketone Reductases from Candida parapsilosis

Abstract

Two conjugated polyketone reductases (CPRs) were isolated from Candida parapsilosis IFO 0708. The primary structures of CPRs (C1 and C2) were analyzed by amino acid sequencing. The amino acid sequences of both enzymes had high similarity to those of several proteins of the aldo-keto-reductase (AKR) superfamily. However, several amino acid residues in the putative active sites of AKRs were not conserved in CPRs-C1 and -C2.

• conjugated polyketone reductase
• aldoketo reductase
• asymmetric reduction
• Candida parapsilosis
• optically active alcohols