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Abstract
A simple method for intra- and interspecific transformation of Bacillus subtilis on solid media has been devised with DNA in protoplast lysates, 0.8% agar, glutamate, and yeast extract. The transformation frequency is 2.3×103 transformants per μg DNA, 10-20 times higher than that for conventional transformation on solid media. The method can be applicable to transformation in microtiter plates.