Characterization of Cloned Mouse Na⁺/taurocholate Cotransporting Polypeptide by Transient Expression in COS-7 Cells

Tohru SAEKILaboratory of Vital & Molecular Nutrition, Department of Biological Resource Chemistry, Faculty of Agriculture, Kyoto Prefectural University Nakaragi, Shimogamo, Sakyo-ku, Kyoto 606-8522, Japan

Noriki TAKAHASHILaboratory of Vital & Molecular Nutrition, Department of Biological Resource Chemistry, Faculty of Agriculture, Kyoto Prefectural University Nakaragi, Shimogamo, Sakyo-ku, Kyoto 606-8522, Japan

Ryuhei KANAMOTOLaboratory of Vital & Molecular Nutrition, Department of Biological Resource Chemistry, Faculty of Agriculture, Kyoto Prefectural University Nakaragi, Shimogamo, Sakyo-ku, Kyoto 606-8522, Japan

Kimikazu IWAMILaboratory of Vital & Molecular Nutrition, Department of Biological Resource Chemistry, Faculty of Agriculture, Kyoto Prefectural University Nakaragi, Shimogamo, Sakyo-ku, Kyoto 606-8522, Japan

Abstract

The mouse Na⁺/taurocholate cotransporting polypeptide transiently expressed in COS-7 cells caused sodium-dependent uptake of [³H]taurocholic acid with K_m and V_max values of 18 μM and 102 pmol/mg protein/min, respectively. This K_m value is comparable to that for rat NTCP and higher than that for human NTCP. Substrate specificity was evaluated by measuring inhibitory effects of unlabeled bile acids on [³H]taurocholic acid transport.

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Characterization of Cloned Mouse Na⁺/taurocholate Cotransporting Polypeptide by Transient Expression in COS-7 Cells

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Characterization of Cloned Mouse Na+/taurocholate Cotransporting Polypeptide by Transient Expression in COS-7 Cells

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Characterization of Cloned Mouse Na⁺/taurocholate Cotransporting Polypeptide by Transient Expression in COS-7 Cells