Green Fluorescent Protein-labeled Recombinant Fluobody for Detecting the Picloram Herbicide

In Seon KIMDepartment of Chemical and Materials Engineering, University of Alberta Edmonton, Alberta T6G 2G6, Canada

Jae Han SHIMDepartment of Biological and Environmental Chemistry, Institute of Agricultural Science and Technology, Chonnam National University Kwang-Ju 500-757, South Korea

Yong Tack SUHDepartment of Biological and Environmental Chemistry, Institute of Agricultural Science and Technology, Chonnam National University Kwang-Ju 500-757, South Korea

Kerrm Y. F. YAUDepartment of Environmental Biology, University of Guelph Guelph, Ontario N1G 2W1, Canada

J. Christopher HALLDepartment of Environmental Biology, University of Guelph Guelph, Ontario N1G 2W1, Canada

Jack T. TREVORSDepartment of Environmental Biology, University of Guelph Guelph, Ontario N1G 2W1, Canada

Hung LEEDepartment of Environmental Biology, University of Guelph Guelph, Ontario N1G 2W1, Canada

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Abstract

A green fluorescent protein-labeled fluobody was designed to develop a simple immunoassay method for detecting picloram herbicide in an environmental sample. The gfp gene was successfully inserted into the pSJF2 vector harboring the picloram-specific antibody fragment to yield pSJF2GFP. Picloram spiking in an environmental river sample could be indirectly detected by observing the fluorescence intensity value of the gfp-fluobody, exhibiting specific sensitivity to free picloram with an IC₅₀ value of 50 ppb. Using the gfp-fluobody immunoassay avoids the enzyme-substrate reaction for calorimetric detection that is required in an enzyme-linked immunosorbent assay (ELISA).

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Green Fluorescent Protein-labeled Recombinant Fluobody for Detecting the Picloram Herbicide

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Green Fluorescent Protein-labeled Recombinant Fluobody for Detecting the Picloram Herbicide

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