Structural Analysis of an Extracellular Polysaccharide Bioflocculant of Klebsiella pneumoniae

Abstract

The glycoside composition and sequence of an extracellular polysaccharide flocculant of Klebsiella pneumoniae H12 was analyzed. GC and HPLC analysis of the acid-hydrolysate identified its constituent monosaccharides as D-Glc, D-Man, D-Gal, and D-GlcA in an approximate molar ratio of 3.9:1.0:2.3:3.6. To analyze the glycoside sequence, the polysaccharide was partially hydrolyzed by acid and enzyme treatment. GC, HPLC, TLC, MALDI-TOF/MS, and 1H- and 13C- NMR spectroscopy characterized the obtained oligosaccharides.

The results clarified the partial structure of H12 polysaccharide as a linear polymer of a unit of pentasaccharide with a side chain of one D-GlcA to D-Glc moiety (see below). Although the existence of other sequences or other constituent glycosides could not be fully excluded, H12 polysaccharide must be a novel types as such a complicated unit for a polymer has not so far been reported. The partial structure of a H12 polysaccharide flocculant is also discussed in this report.

→4)- α-D-Glcp-(1→2)-α-D-Manp-(1→3)-4,6-Pyr-β-D-   3 Galp-(1→4)-β-D-Galp-(1→   ↓

1 β-D-GlcpA

• Klebsiella pneumoniae
• polysaccharide
• oligosaccharides
• flocculant