Efficient Release of Overproduced Gene Products from Escherichia coli BL21(DE3) by Lytic Infection with Newly Isolated Bacteriophages

Yuichiro IIDALaboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University Nara 631-8505, Japan

Yoshinori MATSUDALaboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University Nara 631-8505, Japan

Ryuichiro SAITOLaboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University Nara 631-8505, Japan

Masanori NAKASATOLaboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University Nara 631-8505, Japan

Teruo NONOMURALaboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University Nara 631-8505, Japan

Koji KAKUTANIPharmaceutical Research and Technology Institute, Kinki University Higashi-Osaka 577-8502, Japan

Yukio TOSALaboratory of Plant Pathology, Faculty of Agriculture, Kobe University Kobe 657-8501, Japan

Shigeyuki MAYAMALaboratory of Plant Pathology, Faculty of Agriculture, Kobe University Kobe 657-8501, Japan

Hideyoshi TOYODALaboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University Nara 631-8505, Japan

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Abstract

Overproduced proteins from Escherichia coli BL21(DE3) were efficiently released with virulent bacteriophages. Leviviridae-like bacteriophages were isolated from soil and used to lyse BL21(DE3) cells transformed with β-glucosidase, chitinase, or chitosanase genes. This method caused lysis of bacterial cells similar to that by conventional sonication and enabled us to effectively recover and purify the enzymes.

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Efficient Release of Overproduced Gene Products from Escherichia coli BL21(DE3) by Lytic Infection with Newly Isolated Bacteriophages

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Efficient Release of Overproduced Gene Products from Escherichia coli BL21(DE3) by Lytic Infection with Newly Isolated Bacteriophages

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