Reconstitution and Characterization of NtrC Protein in a Deep-sea Piezophilic Bacterium, Shewanella violacea Strain DSS12

Hiroaki KAWANODepartment of Applied Chemistry, Faculty of Engineering, Toyo University2100 Kujirai, Kawagoe, Saitama 350-0852, Japan;The DEEP STAR Group, Japan Marine Science and Technology Center2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan

Akihiko IKEGAMIDepartment of Oral Biology, State University of New York304 Foster Hall, 3435 Main Street, Buffalo, New York 14214-3092, USA

Kaoru NAKASONEDepartment of Biotechnology and Chemistry, School of Engineering, Kinki University1 Umenobe Takaya, Higashihiroshima, Hiroshima 739-2116, Japan

Chiaki KATOMarine ecosystems Research Department, Japan Marine Science and Technology Center2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan

Ron USAMIDepartment of Applied Chemistry, Faculty of Engineering, Toyo University2100 Kujirai, Kawagoe, Saitama 350-0852, Japan

Koki HORIKOSHIDepartment of Applied Chemistry, Faculty of Engineering, Toyo University2100 Kujirai, Kawagoe, Saitama 350-0852, Japan;The DEEP STAR Group, Japan Marine Science and Technology Center2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan

Abstract

NtrC protein of piezophilic Shewanella violacea was overexpressed and purified, to confirm the protein-DNA interaction. An electrophoretic mobility shift assay demonstrated that the NtrC recognizes the sequence for NtrC binding within the region upstream of the glnA operon. Western blot analysis also showed that the NtrC is expressed at a higher level under high-pressure conditions than under atmospheric pressure conditions.

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Reconstitution and Characterization of NtrC Protein in a Deep-sea Piezophilic Bacterium, Shewanella violacea Strain DSS12

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Reconstitution and Characterization of NtrC Protein in a Deep-sea Piezophilic Bacterium, Shewanella violacea Strain DSS12

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