Construction of a Homologous Selectable Marker Gene for Lentinula edodes Transformation

Toshikazu IRIEIwate Biotechnology Research Center22-174-4 Narita, Kitakami, Iwate 024-0003, Japan

Toshitsugu SATOIwate Biotechnology Research Center22-174-4 Narita, Kitakami, Iwate 024-0003, Japan

Kumiko SAITOIwate Biotechnology Research Center22-174-4 Narita, Kitakami, Iwate 024-0003, Japan

Yoichi HONDAWood Research Institute, Kyoto UniversityGokasho, Uji, Kyoto 611-0011, Japan

Takashi WATANABEWood Research Institute, Kyoto UniversityGokasho, Uji, Kyoto 611-0011, Japan

Masaaki KUWAHARAInstitute of Wood Technology, Akita Prefectural UniversityNoshiro, Akita 016-0876, Japan

Hitoshi ENEIIwate Biotechnology Research Center22-174-4 Narita, Kitakami, Iwate 024-0003, Japan

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Abstract

We cloned a gene for the iron sulfur protein (Ip) subunit from an edible mushroom, Lentinula edodes, and introduced a point mutation that confers carboxin resistance into it. The mutant gene successfully transformed L. edodes with high efficiency (9 transformants/2.5 μg vector DNA). Restriction enzyme-mediated integration (REMI) increased the transformation efficiency by about two-fold.

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Construction of a Homologous Selectable Marker Gene for Lentinula edodes Transformation

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Construction of a Homologous Selectable Marker Gene for Lentinula edodes Transformation

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