A Simple and Efficient Method for High Fidelity PCR Cloning Using Antibody-neutralizing Technology

Abstract

We introduce the TA cloning antibody method for the high-fidelity PCR product amplified by family B DNA polymerase without purification. This method uses antibodies and Thermus aquaticus (Taq) DNA polymerase. The antibodies can inhibit only the activity of family B DNA polymerase, and Taq can co-work for A-tailing. This method has nearly cloning efficiency to that of the PCR product of Taq.

• high-fidelity PCR product
• family B DNA polymerase
• KOD DNA polymerase
• TA cloning method
• antibody