Extracellular Acidic Polysaccharide Production by a Two-membered Bacterial Coculture

Shinya KURATAKankyo Engineering Corporation 1 Kimitsu, Kimitsu-shi, Chiba 299-1141, Japan ;Department of Biomolecular Engineering, Tokyo Institute of Technology 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan

Kazutaka YAMADAKankyo Engineering Corporation 1 Kimitsu, Kimitsu-shi, Chiba 299-1141, Japan

Kyoko TAKATSUKankyo Engineering Corporation 1 Kimitsu, Kimitsu-shi, Chiba 299-1141, Japan

Satoshi HANADAInstitute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST) 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan

Osamu KOYAMAKankyo Engineering Corporation 1 Kimitsu, Kimitsu-shi, Chiba 299-1141, Japan

Toyokazu YOKOMAKUKankyo Engineering Corporation 1 Kimitsu, Kimitsu-shi, Chiba 299-1141, Japan

Yoichi KAMAGATAInstitute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST) 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan

Takahiro KANAGAWADepartment of Biomolecular Engineering, Tokyo Institute of Technology 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan ;Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST) 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan

Ryuichiro KURANEInstitute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST) 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan

show all

Abstract

A two-membered coculture of strains KYM-7 and KYM-8, identified as Cellulomonas cellulans and Agrobacterium tumefaciens, respectively, produced a large amount of an extracellular polysaccharide, designated APK-78, from starch. Each strain in pure culture produced only very little amount of polysaccharide from starch; the coexistence of the two strains from the early stage of cultivation was indispensable for a large amount of polysaccharide to be produced. The polysaccharide APK-78 was acidic and composed of glucose, galactose, succinic acid, and pyruvic acid with a molar ratio of 8.1:1.0:1.7:1.0, indicating that it is a succinoglycan type of polysaccharide.

Keywords

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Extracellular Acidic Polysaccharide Production by a Two-membered Bacterial Coculture

Information for

Open access

Opportunities

Help and information

Extracellular Acidic Polysaccharide Production by a Two-membered Bacterial Coculture

Abstract

Reprints and Corporate Permissions

Academic Permissions

Related research

To cite this article:

Download citation

Information for

Open access

Opportunities

Help and information

Keep up to date

Your download is now in progress and you may close this window

Login or register to access this feature