Design of Soymetide-4 Derivatives to Potentiate the Anti-alopecia Effect

Abstract

Previously, we found that soymetide-4 (MITL), an N-formyl-methionyl-leucyl-phenylalanine (fMLP) agonist peptide derived from soybean β-conglycinin α′ subunit, stimulated phagocytosis of human polymorphonuclear leukocytes, and inhibited alopecia induced by etoposide, an anticancer drug, in neonatal rats after oral administration. We found that the fMLP receptor affinity and phagocytosis-stimulating activity of soymetide-4 was potentiated by replacement of Thr³ with hydrophobic residues. Among the derivatives synthesized, [Trp]³-soymetide-4 (MIWL) was the most potent, stronger by 180 and 130 times than soymetide-4 in receptor affinity and phagocytosis-stimulating activity, respectively. The anti-alopecia effect of [Trp]³-soymetide-4 was about 3 times larger than that of soymetide-4 after oral administration.

• N-formyl-methionyl-leucyl-phenylalanine (fMLP)
• phagocytosis
• alopecia
• soybean
• peptide