Involvement of a Glu71–Arg64 Couple in the Access Channel for NADH in Cytochrome P450nor

Fei SUInstitute of Applied Biochemistry, University of Tsukuba

Shinya FUSHINOBUDepartment of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo

Naoki TAKAYAInstitute of Applied Biochemistry, University of Tsukuba

Hirofumi SHOUNDepartment of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo

Abstract

Putative access channel for NADH in the heme-distal pocket of cytochrome P450nor (P450nor) comprises many charged amino acid residues. Characterization of the E71A mutant protein of P450nor highlights the existence of a unique mechanism for binding NADH that depends on the salt bridge network between Glu71, Arg64 and Asp88.

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Involvement of a Glu71–Arg64 Couple in the Access Channel for NADH in Cytochrome P450nor

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Involvement of a Glu71–Arg64 Couple in the Access Channel for NADH in Cytochrome P450nor

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