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Bioscience, Biotechnology, and Biochemistry Volume 69, 2005 - Issue 9
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Original Articles

Culture-Independent Analysis of Fecal Microbiota in Cattle

Yuhei OZUTSUMIUnited Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology;Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center
,
Hidenori HAYASHIMicrobe Division/Japan Collection of Microorganisms, RIKEN BioResource Center
,
Mitsuo SAKAMOTOMicrobe Division/Japan Collection of Microorganisms, RIKEN BioResource Center
,
Hisao ITABASHIUnited Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology
&
Yoshimi BENNOMicrobe Division/Japan Collection of Microorganisms, RIKEN BioResource Center
Pages 1793-1797 | Received 07 Apr 2005, Accepted 13 Jun 2005, Published online: 22 May 2014
 
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Abstract

The phylogenetic diversity of the fecal bacterial community in Holstein cattle was determined by 16S ribosomal RNA gene sequence analysis. The sequences were affiliated with the following phyla: Firmicutes (81.3%), Bacteroidetes (14.4%), Actinobacteria (2.5%), and Proteobacteria (1.4%). The Clostridium leptum subgroup was the most phylogenetically diverse group in cattle feces. In addition, a number of previously uncharacterized and unidentified bacteria were recognized in clone libraries.

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