Identification of 2-(Cysteinyl)amido-2-deoxy-D-galacturonic Acid Residue from the Sheath of Leptothrix cholodnii

Hiroko MAKITADivision of Materials Science and Chemical Engineering, Faculty of Engineering, Yokonama National University

Yuichi NAKAHARADivision of Materials Science and Chemical Engineering, Faculty of Engineering, Yokonama National University

Hiroyuki FUKUIDivision of Materials Science and Chemical Engineering, Faculty of Engineering, Yokonama National University

Yohei MIYANOIRIDepartment of Supramolecular Biology, International Graduate School of Arts and Sciences, Yokohama City University

Masato KATAHIRADepartment of Supramolecular Biology, International Graduate School of Arts and Sciences, Yokohama City University;Genome Science Center, RIKEN;PRESTO, JST

Hiroko SEKIChemical Analysis Center, Chiba University

Minoru TAKEDADivision of Materials Science and Chemical Engineering, Faculty of Engineering, Yokonama National University

Jun-ichi KOIZUMIDivision of Materials Science and Chemical Engineering, Faculty of Engineering, Yokonama National University

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Abstract

The sheath of Leptothrix cholodnii is a glycoconjugate composed of a polysaccharide and a peptide rich in cysteine. In this study, structural determination of the hydrazinolyzate of the sheath was carried out. Since the hydrazinolyzate is a polysaccharide incorporated with cysteine, it was S-derivatized with a thiol-specific fluorogenic reagent, 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (ABD-F). Fluorescent fragments were purified by HPLC, and their structures were analyzed by mass spectrometry and NMR spectroscopy. The sheath was found to contain 2-(cysteinyl)amido-2-deoxy-D-galacturonic acid residue.

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Identification of 2-(Cysteinyl)amido-2-deoxy-D-galacturonic Acid Residue from the Sheath of Leptothrix cholodnii

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Identification of 2-(Cysteinyl)amido-2-deoxy-D-galacturonic Acid Residue from the Sheath of Leptothrix cholodnii

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