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Bioscience, Biotechnology, and Biochemistry Volume 71, 2007 - Issue 8
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Original Articles

Improved Gateway Binary Vectors: High-Performance Vectors for Creation of Fusion Constructs in Transgenic Analysis of Plants

Tsuyoshi NAKAGAWADepartment of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
,
Takamasa SUZUKIDepartment of Biological Functions and Mechanisms, Graduate School of Bioagricultural Sciences, Nagoya University
,
Satoko MURATADepartment of Biological Functions and Mechanisms, Graduate School of Bioagricultural Sciences, Nagoya University
,
Shinya NAKAMURADepartment of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
,
Takeshi HINODepartment of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
,
Kenichiro MAEODepartment of Biological Functions and Mechanisms, Graduate School of Bioagricultural Sciences, Nagoya University
,
Ryo TABATADepartment of Biological Functions and Mechanisms, Graduate School of Bioagricultural Sciences, Nagoya University
,
Tsutae KAWAIDepartment of Biological Functions and Mechanisms, Graduate School of Bioagricultural Sciences, Nagoya University
,
Katsunori TANAKADepartment of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University
,
Yasuo NIWALaboratory of Plant Cell Technology, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka
,
Yuichiro WATANABEDepartment of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo
,
Kenzo NAKAMURADepartment of Biological Functions and Mechanisms, Graduate School of Bioagricultural Sciences, Nagoya University
,
Tetsuya KIMURADepartment of Sustainable Resource Science, Faculty of Bioresources, Mie University
&
Sumie ISHIGURODepartment of Biological Functions and Mechanisms, Graduate School of Bioagricultural Sciences, Nagoya University
 show all
Pages 2095-2100 | Received 16 Apr 2007, Accepted 21 Jun 2007, Published online: 22 May 2014
 
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Abstract

We made a series of improved Gateway binary vectors (pGWBs) for plant transformation. Fifteen different reporters and tags, sGFP, GUS, LUC, EYFP, ECFP, G3GFP, mRFP, 6×His, FLAG, 3×HA, 4×Myc, 10×Myc, GST, T7, and TAP, were employed. Some vectors carry the 2×35S-Ω promoter for higher-level expression. The kanamycin- and hygromycin-resistant markers are independently available for each of the 43 types of vectors, thus an additional transformation of once-transformed plants can be carried out easily. Their small size and high-copy number in Escherichia coli make possible easier handling at plasmid preparation and sequencing. Improved pGWBs should be a powerful tool for transgenic research in plants.

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