Species-Specific Detection of Listeria monocytogenes Using Polymerase Chain Reaction Assays Targeting the prfA Virulence Gene Cluster

Hye-Jin JUNGDepartment of Food Science and Technology, College of Industrial Sciences, Chung-Ang University

Sung-Hee PARKDepartment of Food Science and Technology, College of Industrial Sciences, Chung-Ang University

Sang-Do HADepartment of Food Science and Technology, College of Industrial Sciences, Chung-Ang University

Kyu-Ho LEEDepartment of Environmental Engineering and Biotechnology, Hankuk University of Foreign Studies

Duck Hwa CHUNGDivision of Applied Life Science, Gyeongsang National University

Cheol-Ho KIMDepartment of Biological Sciences, College of Natural Science, Sungkyunkwan University

Min-Gon KIMLaboratory of Integrative Biotechnology, Korea Research Institute of Bioscience and Biotechnology

Kwang-Yup KIMDepartment of Food Science and Technology, Chungbuk National University

Keun-Sung KIMDepartment of Food Science and Technology, College of Industrial Sciences, Chung-Ang University

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Abstract

Sequence comparison of individual genes in the prfA virulence gene cluster (pVGC), a central virulence gene cluster, from different Listeria species indicated that priming sites within the genes appeared to be specific for Listeria monocytogenes exclusively. Therefore, the pVGC was targeted for polymerase chain reaction (PCR) assays to detect and specifically identify L. monocytogenes. Each gene of the pVGC was specifically amplified in L. monocytogenes but not in other Listeria species.

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Species-Specific Detection of Listeria monocytogenes Using Polymerase Chain Reaction Assays Targeting the prfA Virulence Gene Cluster

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Species-Specific Detection of Listeria monocytogenes Using Polymerase Chain Reaction Assays Targeting the prfA Virulence Gene Cluster

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