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Original Articles

Yeast Cell-Surface Expression of Chitosanase from Paenibacillus fukuinensis

, , , , , , & show all
Pages 2845-2847 | Received 21 May 2007, Accepted 02 Aug 2007, Published online: 22 May 2014
 

Abstract

To produce chitoorigosaccharides using chitosan, we attempted to construct Paenibacillus fukuinensis chitosanase-displaying yeast cells as a whole-cell biocatalyst through yeast cell-surface engineering. The localization of the chitosanase on the yeast cell surface was confirmed by immunofluorescence labeling of cells. The chitosanase activity of the constructed yeast was investigated by halo assay and the dinitrosalicylic acid method.

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