High-Level Expression of Recombinant Active Human Renin in Sf-9 Cells: Rapid Purification and Characterization

Saori TAKAHASHIInstitute for Food and Brewing, Akita Prefectural Agricultural, Forestry, and Fisheries Research Center

Keishi HATAInstitute for Food and Brewing, Akita Prefectural Agricultural, Forestry, and Fisheries Research Center

Ken-Ichi KIKUCHIDepartment of Materials-Process Engineering and Applied Chemistry for the Environment, Akita University

Takeshi GOTOHDepartment of Materials-Process Engineering and Applied Chemistry for the Environment, Akita University

Abstract

Recombinant human (rh) renin was expressed in Sf-9 insect cells. Baculovirus-infected Sf-9 cells produced active rh-renin in the late stage of cultivation. The rh-renin was purified after 5 d of culture by two steps of column chromatography. Approximately 0.61 mg of pure rh-renin was obtained from 200 ml of culture medium with a yield of 35.3%.

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High-Level Expression of Recombinant Active Human Renin in Sf-9 Cells: Rapid Purification and Characterization

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High-Level Expression of Recombinant Active Human Renin in Sf-9 Cells: Rapid Purification and Characterization

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