Physicochemical Properties of Diheme Cytochrome c₄ of Unknown Function from Vibrio parahaemolyticus Strain RIMD2210633

Hideharu AKAZAKIBio-Organic Chemistry Laboratory, Graduate School of Bioresource Sciences, Nihon University

Yoshio FUTAMIBio-Organic Chemistry Laboratory, Graduate School of Bioresource Sciences, Nihon University

Naoya SHIBAYAMADepartment of Physiology, Division of Biophysics, Jichi Medical School

Ikuko SHIRASAKIBio-Organic Chemistry Laboratory, Graduate School of Bioresource Sciences, Nihon University

Harumi NAKADEBio-Organic Chemistry Laboratory, Graduate School of Bioresource Sciences, Nihon University

Hirotaka CHIDABio-Organic Chemistry Laboratory, Graduate School of Bioresource Sciences, Nihon University

Wataru HAKAMATABio-Organic Chemistry Laboratory, Graduate School of Bioresource Sciences, Nihon University

Sam-Yong PARKProtein Design Laboratory, Guraduate School of Integrated Science, Yokohama City University

Toshiyuki NISHIOBio-Organic Chemistry Laboratory, Graduate School of Bioresource Sciences, Nihon University

Tadatake OKUBio-Organic Chemistry Laboratory, Graduate School of Bioresource Sciences, Nihon University

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Abstract

To characterize a diheme cytochrome c ₄ of unknown functional of the Vibrio genus for the first time, the Vibrio parahaemolyticus cytochrome c ₄ was overexpressed in Escherichia coli periplasm using the endogenous signal sequence. The physicochemical properties of the purified recombinant protein, viz., molecular mass, UV/Vis, and CD spectra, and the redox potentials of the N- and C-terminal domain hemes were determined.

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Physicochemical Properties of Diheme Cytochrome c₄ of Unknown Function from Vibrio parahaemolyticus Strain RIMD2210633

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Physicochemical Properties of Diheme Cytochrome c₄ of Unknown Function from Vibrio parahaemolyticus Strain RIMD2210633