Isolation of Herbicide-Resistant 4-Hydroxyphenylpyruvate Dioxygenase from Cultured Coptis japonica Cells

Abstract

4-Hydroxyphenylpyruvate dioxygenase (HPPD) catalyzes the formation of homogentisate from 4-hydroxyphenylpyruvate and O₂. In plants, HPPD has been identified as a molecular target for herbicides. We report the isolation and characterization of a cDNA encoding a HPPD from cultured Coptis japonica cells. Recombinant CjHPPD showed significantly higher half-maximum inhibitory concentration (IC₅₀) values for the HPPD-inhibiting herbicide destosyl pyrazolate than other plant HPPDs.

• 4-hydroxyphenylpyruvate dioxygenase
• Coptis japonica
• destosyl pyrazolate
• benzobicyclon hydrolysate
• herbicide resistance