Development of a Homologous Transformation System for Aspergillus aculeatus Based on the sC Gene Encoding ATP-Sulfurylase

Abstract

A homologous transformation system was developed using the endogenous ATP-sulfurylase gene, AasC, as a selectable marker in Aspergillus aculeatus. Spontaneous mutation was proved to be beneficial in isolating AasC-deficient mutants. Molecular analysis of sC ⁺ transformants revealed that the frequency of single copy integration at ATP-sulfurylase locus was more than 40%.

• Aspergillus aculeatus
• ATP-sulfurylase gene
• homologous transformation
• two-way selection