Genes Encoding Carbocycle-Forming Enzymes Involved in Aminoglycoside Biosynthesis in Deep-Sea Environmental DNA

Abstract

We obtained 19 individual DNA fragments encoding 2-deoxy-scyllo-inosose synthase involved in the biosynthesis of aminoglycoside antibiotics from deep-sea sediments of the Pacific Ocean. Compared with genes from land-based environmental DNA, they showed low diversity. Combined with our previous study concerning the discovery of other aminoglycoside-biosynthetic genes from the same deep-sea samples, we suggest the importance of exploration of multiple biosynthetic genes to determine the diversity of aminoglycoside producers. We found that the deep sea is a useful source for screening of these genes.

• deep sea
• aminoglycoside
• biosynthetic gene
• 2-deoxy-scyllo-inosose synthase
• environmental DNA