Expression, Purification, Physicochemical Characterization and Structural Analysis of Cytochrome c₅₅₄ from Vibrio parahaemolyticus Strain RIMD2210633

Abstract

The function of cytochrome c ₅₅₄ of Vibrio parahaemolyticus has not yet been determined. We have determined the physicochemical properties and crystal structure of cytochrome c ₅₅₄ at 1.8 Å in order to help elucidate its function. The physicochemical properties and the tertiary structure of cytochrome c ₅₅₄ resemble those of dimeric cytochrome c ₅₅₂ from Pseudomonas nautica, but the Vibrio genus contains no gene for nitrite reductase, cytochrome cd ₁, in its genome DNA. These results raise the possibility that both cytochromes denote an electron to an electron carrier and accept an electron from same electron carrier.

• cytochrome c ₅₅₄
• crystal structure
• Vibrio parahaemolyticus
• physicochemical property