Isolation Columns as an Alternative to Replicate Ponds in Aquaculture Research

Abstract

The use of open-ended isolation columns (20 m³) as replicable experimental units was evaluated in two Alabama Piedmont ponds. Isolated columns of water differed limnologically from conditions present in the open ponds. Most of these differences were attributed to open pond sites having significantly higher phytoplankton biomass (chlorophyll-a). However, comparison of pond and column variances indicated that samples collected in columns were generally no more indicated that samples collected in columns were generally no more variable than similar samples collected from the pond that surrounded them. Isolation columns could be used as an alternative to replicate ponds for certain types of short-term, pond culture studies.