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Abstract
The objective of this study was to analyze channel catfish feeds for folic acid and pteroic acid concentrations and correlate these substances with anemia in channel catfish, Ictalurus puncratus. From 1983 to 1987, feed-related anemia was diagnosed 298 times in cultured channel catfish in Alabama. Analysis of eight brands of anemia-associated feeds (AAF) and nonanemia-associated feeds (NAAF) suggested that AAF were either lower in folic acid or were higher in pteroic acid concentrations than the NAAF. AAF contained a folic acid: pteroic acid ratio of less than 1.8:1, compared to a folic acid:pteroic acid ratio of greater than 10:1 in NAAF. Low folic acid concentrations were due either to insufficient addition at the feed mill or due to degradation during storage.