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Journal of Applied Aquaculture Volume 11, 2001 - Issue 3
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Original Articles

Development of an Indirect ELISA to Detect Humoral Response to Strep to coc cus iniae Infection of Nile Tilapia, Oreochromis niloticus

Richard A. Shelby Aquatic An i mal Health Research Laboratory, Agricultural Re search Ser vice, U.S. De part ment of Ag ricul ture, P.O. Box 952, Au burn, AL, 36830, USA
,
Craig A. Shoemaker Aquatic An i mal Health Research Laboratory, Agricultural Re search Ser vice, U.S. De part ment of Ag ricul ture, P.O. Box 952, Au burn, AL, 36830, USA
,
Joyce J. Evans Aquatic Animal Health Research Laboratory, Agricultural Research Service, U.S. De part ment of Ag ri cul ture, 300 Wash ing ton Av e nue, Chestertown, MD, 21620, USA
&
Phillip H. Klesius Aquatic An i mal Health Research Laboratory, Agricultural Re search Ser vice, U.S. De part ment of Ag ricul ture, P.O. Box 952, Au burn, AL, 36830, USA
Pages 35-44 | Published online: 14 Aug 2009
 
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Abstract

The humoral an ti body of Nile tilapia, Oreochromis niloticus, to Strep to coc cus iniae in fec tion was mea sured in two groups by an in di rect en zyme-linked immunoassay (ELISA). One treat ment group was ex per i men tally in oc u lated with S. iniae, and the other con sisted of a non-in oc u lated con trol. The an ti body re sponse of the ex per i men tal infected group was sig nif i cantly dif fer ent from the non-in fected tilapia. The in di rect ELISA uti lized goat anti-tilapia IgM and a com mer cially avail able rab bit anti-goat IgG en zyme con ju gate. The spec i fic ity of the an ti body re sponse was di rected against cell-free sonicated an ti gen prepa ra tion from whole S. iniae ARS-98-60 cells. The ELISA re sults were highly cor re lated with the ag glu ti na tion re sults for tilapia anti- S. iniae an ti body.

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Related Research Data

The Efficacy of Nile Tilapia (Oreochromis niloticus) Broodstock and Larval Immunization against Streptococcus agalactiae and Aeromonas hydrophila
Source: Multidisciplinary Digital Publishing Institute
Generation of mouse monoclonal antibodies specific to tilapia immunoglobulin using fish immunoglobulin/BSA complex for monitoring of the immune response in Nile tilapia Oreochromis niloticus
Source: Wiley

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