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Abstract
The work was conducted to optimize a procedure of gynogenesis induction in the stinging catfish, Heteropneustes fossilis by suppressing meiotic cell division in fertilized eggs. Gynogenesis was induced by fertilizing normal eggs with genetically inactivated sperm followed by cold-shocking. The sperm were inactivated by exposing them to aUV dose of 250 μw/cm2for2.5 minutes at a concentration of 1 × 108sperm/mL. The optimum cold-shock condition to produce maximum percentage of gynogens was found to be at 2°C for 10 or 15 minutes initiated within 3-7 minutes of fertilization. Karyological examination of one day-old larvae and comparison of the erythrocyte area and volume of 3 month-old putative gynogen fry with those of normal fry were used to confirm the success of gynogenesis induction. The findings of the present work are expected to open up avenues for production of diploid gynogens and creation of monosex female populations in stinging catfish.