Micropropagation of Plumbago zeylanica Linn

ABSTRACT

A rapid and highly effective method for plant micro-propagation from vegetative shoot buds was established for the medicinal plant Plumbago zeylanica Linn. Multiple shoots were proliferated from nodal explants cultured on Murashige and Skoog basal medium supplemented with kinetin or N⁶-benzyl adenine (0.25–1.0 mg/l). Excised shootlets cultured on hormone-free basal medium rooted within 4 to 7 days. Successful transfer of the rooted shoots to potting soil has been accomplished with 100 percent survival.

KEYWORDS:

• Medicinal plant
• tissue culture
• propagation