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SUMMARY
Breeding research for improvement of the homogeneity of marjoram pollinator lines requires reliable information on the genetic diversity. Three progenies of marjoram pollinator lines were examined by means of RAPD assays. For this reason a suitable protocol for DNA isolation and the conditions for the PCR reactions were worked out. A single plant analysis as well as an analysis with bulked DNA samples were used. The RAPD fingerprints of single plants illustrate the heterogeneity inside the accessions in the first year and the enhancement of the homogeneity in the next progenies causing by selfing. For revealing the genetic distances of the accessions, the pooling strategy is more suitable than the single plant analysis. The simplicity of the RAPDs makes it possible to realize a high number of analyses, but the results should be evaluated with caution, with knowledge about some drawbacks of the method. Nevertheless, there is the conclusion that RAPDs are a useful tool in marjoram breeding programs.
