SUMMARY
Cultivation media for the production of both a cellulase containing and a cellulase-free polygalacturonase (PG) preparation by a highly productive strain of Sclerotium rolfsii (CBS 350.80) were developed. These enzyme preparations both contained a PG activity higher than 500 IUmL−1 and were applied in a bioscouring sequence of knitted cotton at various activity levels. The progress of bioscouring was evaluated by weight loss, water absorption time and determination of residual pectin. Released sugars have been determined and compared to bioscouring effects. No significant differences among the tested preparations were obtained for water absorption and pectin removal. However, considerably higher weight losses were observed for the preparations containing cellulase. A minimal enzyme dosage of 100 IUg−1 cotton was necessary to achieve suitable scouring results under the applied conditions.