Antifibrinolytic/Recombinant PAI-1: An Assay for the Detection of Inhibitors of the Interaction between PAI-1 and tPA

Summary

Novelty: Biologically functional mature E. coli expressing recombinant PAI-1 are disclosed, as are plasmid expression vectors for the production of rPAI-1, and a method for identifying inhibitors of the binding of PAI-1 and tPA.

Biology: Human PAI-1 is expressed in E. coli in a non - fused mature biologically functional form with a specific activity of 0.3 U/ng. Soluble, active human PAI-1 is expressed in E. coli by a recombinant plasmid expression vector and the DNA coding for PAI-1 is operatively linked to a phage lambda or tac promoter. E. coli strain TAP106 is transformed with such a plasmid. A pharmaceutical composition of rPAI-1 is therapeutically effective to counteract excessive or inappropriate fibrinolysis.