![Sample our Bioscience journals, sign in here to start your access, Latest two full volumes FREE to you for 14 days]({"type":"image" , "src" : "/sda/5925/TOC-Subject-Sample-2021-Bioscience.jpg"})
Summary
Novelty: The purification of LAM (lipoarabinomannan) from Mycobacteria in commercially useful quantities is described. Claimed methods involve ion-exchange separation and HPLC rather than preparative gel electrophoresis. Synthetically produced portions of LAM are also claimed. These compounds may facilitate structural elucidation and determination of mechanism of action of LAM, leading to potential treatment of mycobacterial infections.
Biology: No biological data are presented.
Chemistry: The generation of purified LAM from M. tuberculosis is fully described. Final purification was achieved on Sephacryl S-200 columns. Procedures for determining structural formation of the antigenic portion of LAM are provided; twenty-five chemical degradation products are tabulated. Examination of these fragments allowed four families of oligoarabinosides and four major structural motifs to be recognized. The chemical syntheses of four arabinose-containing epitopes of LAM from M. tuberculosis are specifically claimed.