Patent Evaluation: Expression of Mature PDGF-B in Escherichia Coli

Summary

Novelty: An E. coli strain transformed with a recombinant DNA vector, able to express mature active human platelet derived growth factor-B (PDGF-B) chain, is disclosed.

Biology: A mitogenesis assay was performed using human fibroblasts and the in vitro uptake of thymidine was determined. The PDGF-B expressed by E. coli was shown to be at least as active as commercially available PDGF. Radioligand receptor binding assays were also performed and similar results were obtained.

Chemistry: A lon⁻, htpR⁻ E. coli bacteria culture is transformed with a recombinant DNA vector encoding mature human PDGF-B, and the expressed active PDGF-B purified from the culture. Full sequence details are presented.