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Summary
Novelty: A thirty-five residue polypeptide is disclosed which stimulates production of growth hormone and prolactin by pituitary cells. The polypeptide is isolated from thymosin fraction 5 and is identical to a region of histone 2A. It has potential use in the treatment of dwarfism, osteoporosis, ovarian dysgenesis and diffuse gastric bleeding. Stimulation of the production of endogenous growth hormones may be more effective in the treatment of dwarfism than administration of a single species of genetically engineered protein.
Biology: Fractions of HPLC eluate (10μg/ml) were screened for stimulation of GH and PRL release from rat pituitary cells. The specific activity of the final product (ng of hormone produced/μg of peptide) was 226 for PRL and 98 for GH. Co-incubation with 100 nM TRH produced an additive increase in PRL release.
Chemistry: Purification of the claimed polypeptide by two step reverse phage HPLC of TF-5 is described; the yield was 0.1%. SDS-PAGE analysis indicated a molecular weight of 3756 D. Amino acid analysis and sequencing were performed by standard techniques.
Structure:
Ala-lle-Arg-Asn-Asp-Glu-Glu-Leu-Asn-Lys-Leu-Leu-Gly-Lys-Val-Thr-lle-Ala-Gln-Gly-lly-Val-Leu-Pro-Asn-lle-Gln-Ala-Val-Leu-Leu-Pro-Lys-Lys-Thr