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Summary
Novelty: A purified antigen from T. gondii, molecular weight 54kDa, is described. It is claimed to be useful for diagnosis and vaccine production.
Biology: Examples detail the identification of the antigen through cloning of their cDNA in bacterial expression vectors, screening the library with sera from immune subjects, and testing the whole set of antibody-preselected recombinant antigens for reactivity to a T cell clone from the immune subject.
Chemistry: The characterization of the DNA coding sequence, the deduced amino acid sequence of the antigen together with its expression in E. coli using the IGAL12 plasmid vector, is also described.