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Abstract
Novelty: A human serotonin receptor of the 5-HT1E subtype is claimed. The cloned receptor could be used for the screening of novel agonists or antagonists. Serotonin and serotonin receptors are involved in many functions in the body including behavioural functions, pain perception, sleep, aggression and sexual activity.
Biology: A human placental genomic library was screened using oligonucleotides derived from the known sequence of the 5-HT1Dβ receptor gene. DNA sequencing of the positive clones showed one to be a different sub-class to those already known. DNA sequences of this clone were used as probes in the screening of a cDNA library and an expression vector expressing the 5-HT1E receptor in transfected Y1 cells showed an increase in CAMP production after pargyline incubation. The disclosure also claims antibodies specific for this receptor which could be used in analysis of tissues or in drug development. Antisense RNA is claimed which could be used to inhibit the expression of this class of receptor in neuronal tissue.