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Summary
Novelty: A procedure for detecting proteins in fluids, which involves contacting such fluid with a composition comprising copper, is disclosed. The interference of components in certain body fluids with protein determination is reduced or eliminated. Detection of protein in biological fluids such as blood serum and urine is useful in the diagnosis of various abnormalities.
Biology: Biological fluids contain components which interfere with the redox indicator. Thus, a composition containing ionizable phosphate is added to the system to prevent such interference.
Chemistry: The method involves contacting a fluid suspected of containing proteinaceous material with copper, in a form capable of forming a copper/protein complex, a peroxide and a redox indicator which produces a detectable response when oxidised by the peroxide in the presence of the copper/protein complex. The ionizable phosphate to prevent interference is specified.