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Summary
Novelty: Humanized chimaeric monoclonal antibodies against ganglioside GD3 are disclosed with a process for their production. Anti-GD3 antibodies may be useful in the treatment of cancers such as melanoma, in which GD3 is over-expressed.
Biology: No difference in the binding specificity of chimaeric anti-GD3 antibody compared with that of the murine equivalent was detected. The in vitro complement-dependent cytotoxicity and the antibody-dependent cell mediated cytotoxicity of the chimaeric antibody against SK MEL-28 and G361 melanoma cell lines was found to be greater than the murine equivalent. In vivo antitumour activity was determined against G361 cells transplanted into mice. Chimaeric antibody administered iv completely inhibited tumour growth.
Chemistry: The humanized chimaeric antibody is based on the murine antibody KM641 (EP-0493686-A). The method of producing the chimaeric antibody is described. Two polypeptide and DNA sequences are specifically claimed.