11
Views
0
CrossRef citations to date
0
Altmetric
Miscellaneous

Normal neural epithelial precursor cells

Pages 85-89 | Published online: 25 Feb 2005
 

Abstract

The present invention describes a method of isolating non-transformed neural epithelial precursor (NEP) cells capable of continuous replication or differentiation depending on their culture conditions. Isolated neural epithelium from zero somite stage (E9) rat embryos is cultured on laminin in hormone and growth factor supplemented DME/F12 basal medium in the presence of bovine pituitary extract and a Schwann cell conditioned medium containing heregulin and forskolin. NEP cells undergo expansion without a growth crisis, senescence or significant spontaneous differentiation, and retain pluripotency. The authors claim the above method as applied to rodent or human neuroepithelium and compositions including such cells combined in a pharmaceutically acceptable delivery vehicle. Expanded NEP cells are capable of differentiating into neurones expressing central nervous system (CNS) and peripheral nervous system (PNS) markers and glial cell types both in vitro and when implanted into neonatal rat brain.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.