Abstract
The RAPD methodology was utilized for analyzing the genetic material of four all opatric populations of the Ophrys bertoloniiformis type, as well as of two populations of O. bertolonii s.str., together with O. fuciflora as out group. Significant gene diversity was observed for all six taxa under examination, thus suggesting their separation at specific (or sub specific) level. Comparison with other methods (biostatistical analysis and pollination biology) is also reported.