Intestinal homeostasis and neoplasia studied using conditional transgenesis

Abstract

Constitutive mouse models of intestinal neoplasia, such as the Apc^min/+ (multiple intestinal neoplasia) mouse have proven valuable tools both for furthering our understanding of tumorigenesis and for the development of therapeutic strategies. However, the in vivo study of a number of genes has been precluded by their absolute requirement during embryonic development. This has led to the development of conditional strategies that allow gene regulation in vivo. This review describes the principal techniques used to achieve conditional transgenesis within the mouse intestine, with a particular focus upon the Cre–Lox and Tet-regulable systems. Further, we discuss how these techniques are being used to dissect the mechanisms governing both normal homeostasis and neoplastic development within the intestine.

Keywords::

• bone morphogenic protein
• conditional
• Cre–Lox
• intestine
• mouse
• neoplasia
• Notch
• Wnt