Promotion of cancer metastasis: candidate validation using an iTRAQ-based approach

Abstract

Evaluation of: Ghosh D, Li Z, Tan XF, Lim TK, Mao Y, Lin Q. RAQ based quantitative proteomics approach validated the role of calcyclin binding protein (CacyBP) in promoting colorectal cancer metastasis. Mol. Cell Proteomics 12(7), 1865–1880 (2013).

The use of iTRAQ-based relative quantification is demonstrated by Ghosh et al. to analyze the downstream effectors of a calcyclin-binding protein, which is proposed to promote colorectal cancer progression. These findings are reviewed and discussed in relation to the need to establish robust in vitro model cell lines for identification of cancer-specific pathways and to confirm the leads generated by proteomic analysis.

Keywords::

• biomarker
• colorectal cancer
• iTRAQ
• protein network analysis
• relative quantification

Financial & competing interest disclosure

The authors have no relevant affiliations or financial involvement with any organisation or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending or writing.

No writing assistance was utilized in the production of this manuscript.