Blood doping: present procedures and detection techniques

Abstract

Blood doping represents a serious risk in endurance athletes. Blood transfusion practices (either autologous or homologous) have been used since 1960 and, despite the significant improvement in the laboratory methods, only homologous blood transfusion can be detected currently, while for autologous blood transfusion, no validated methods exist. In the last 15 years, a number of drugs have been developed to treat anemic patients. From recombinant erythropoietin to synthetic hemoglobin, all the developed tools are potentially useful to increase the oxygen transport to peripheral tissues in endurance athletes. Thus, the availability of doping-detection methods can only be sustained by the knowledge of any novel therapeutic approach in this field. The identification of the doping molecule is the gold standard of any antidoping campaign; despite this, indirect methods based on the detection of the effects induced by the doping procedure will be a very powerful tool in the near future. Nevertheless, while direct methods are only affected by the sensitivity and the specificity of the method itself (deterministic methods), indirect approaches are affected by the statistic weight of the results (probabilistic methods). Thus, blood doping will be better controlled by the combination of the two approaches.

Keywords::

• blood doping
• blood transfusion
• endurance
• EPO
• indirect tests