Proteomics of blood and derived products: what’s next?

Abstract

Proteomics has changed the way proteins are analyzed in living systems. This approach has been applied to blood products and protein profiling has evolved in parallel with the development of techniques. The identification of proteins belonging to red blood cell, platelets or plasma was achieved at the end of the last century. Then, the questions on the applications emerged. Hence, several studies have focused on problems related to blood banking and products, such as the aging of blood products, identification of biomarkers, related diseases and the protein–protein interactions. More recently, a mass spectrometry-based proteomics approach to quality control has been applied in order to offer solutions and improve the quality of blood products. The current challenge we face is developing a closer relationship between transfusion medicine and proteomics. In this article, these issues will be approached by focusing first on the proteome identification of blood products and then on the applications and future developments within the field of proteomics and blood products.

Keywords::

• plasma
• platelets
• proteomics
• quality control
• red blood cells
• transfusion medicine

Financial & competing interests disclosure

The authors thank the Fondation Humanitaire de la Croix–Rouge Suisse, la Commission de Recherche de Transfusion CRS Suisse and the Télémaque foundation for financial support. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Notes

AQUA: Absolute quantification; BN: Blue native; CE: Capillary electrophoresis; CID: Collision-induced dissociation; ECD: Electron-capture dissociation; ETD: Electron-transfer dissociation; FTICR: Fourier transform ion cyclotron resonance; HILIC: Hydrophilic interaction liquid chromatography; ICAT: Isotope-coded affinity tags; IEF: Isoelectric focusing; IMAC: Immobilized metal ion affinity chromatography; IT: Ion trap; iTRAQ: Isobaric tags for relative and absolute quantification; LC: Liquid chromotography; MRM: Multiple reaction monitoring; MS: Mass spectrometry; m/z: Mass-to-charge ratio; pI: Isoelectric point; Q: Quadrupole; RP: Reversed-phase; SCX: Strong cation exchange; SEC: Size-exclusion chromatography; SRM: Single reaction monitoring; TMT: Tandem mass tag; u: Unified atomic mass.

LC: Liquid chromatography; MS/MS: Tandem mass spectrometry; RP: Reversed phase; SCX: Strong cation exchange.

MS: Mass spectrometry; PLT: Platelet; QALY: Quality-adjusted life year.