https://orcid.org/0000-0003-4326-1991
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Abstract
Aim: Ovarian cancer is a serious malignancy with high prevalence and mortality. Methods: We isolated and characterized an ovarian high-grade serous cancer cell line (M4) from a tumor of a Vietnamese patient with ovarian carcinoma. Results: The M4 cancer cell line showed good proliferation and stability in culture. Morphologically, the M4 cells showed similar characteristics to tumor cells such as a polyhedral shape, large irregular nuclei, high nuclear/cytoplasmic ratio, high nuclear density and expressing cancer markers like CA125, p53 and Ki67 markers. Conclusion: We have successfully isolated and characterized the M4 cell line from a Vietnamese patient with ovarian carcinoma.
Author contributions
NL Toan, HV Tong, and DT Chung designed the study. NT Linh, NT Hang, BK Cuong, LT Chinh and CV Mao were involved in in vitro experiments, animal recruitment and sample collection. NL Toan, HV Tong, DT Chung, NT Linh, NT Hang, BK Cuong, NV Do, TN Dung and CV Mao contributed to materials and reagents and revised the manuscript. NT Linh, NT Hang, and BK Cuong performed the statistical analyses. NT Linh, NT Hang, BK Cuong, HV Tong and NL Toan wrote the first draft and revised the manuscript. All authors read and approved the final manuscript.
Acknowledgments
The authors are grateful to their subjects for their participation in this study.
Financial & competing interests disclosure
All authors gratefully acknowledge the financial support from the key scientific program state-level, Ministry of Science and Technology, Vietnam, code: KC10 /16-20 under grant number KC10.27/16-20. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.
No writing assistance was utilized in the production of this manuscript.
Ethical conduct of research
The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations. In addition, for investigations involving human subjects, informed consent has been obtained from the participants involved.