GC–MS Based Metabolite profiling, Antioxidant and Antiurolithiatic Properties of Apple Cider Vinegar

Abstract

Aim: To perform in vitro analysis of antioxidant and antiurolithiasis to carry out GC–MS-based metabolite profile. Materials & methods: The effect of apple cider vinegar (ACV) in vitro, antioxidant and GC–MS analysis was evaluated. The antioxidant studies were performed. In vitro techniques included nucleation, aggregation and growth assay. Results & conclusion: The presence of polyphenols, flavonoids, alkaloids and carbohydrates was shown. Concentrations from 5–30 μg/ml could dissolve calcium oxalate (p < 0.05) in vitro. The IC₅₀ value of ACV in DPPH was found to be around 7 μg/ml and the IC₅₀ value of the ACV in ABTS assay was around 9 μg/ml. Different phytocompounds were obtained from GC–MS analysis. ACV can be consumed to prevent kidney stones which seems helpful to the current therapy.

Plain Language Summary

Due to the modern lifestyle, urolithiasis continues to be a global medical burden and is getting worse every day and seems to have a very high recurrence rate. Urolithiasis can be managed with the pharmacotherapy of drugs and the usage of medicinal plants and nutraceuticals. The effect of apple juice products on urine physiology has been studied profoundly. The product thus obtained was examined for antioxidant assay using DPPH and in vitro antiurolithiasis activity. Apple cider vinegar (ACV) exhibited dose-dependent radical scavenging activity against free radicals. The ACV demonstrated a substantial suppression of the DPPH radical in the DPPH assay. ACV could be a potential source of a natural antioxidant drink based on in vitro, phytochemical and GC–MS analysis which was noteworthy and thus can be used as a therapeutic agent against bladder stones.

Keywords:

• antioxidant
• antiurolithiatic
• apple cider vinegar
• DPPH
• phytochemical constituents

Author contributions

AS Singh, A Singh, C Vellapandian, R Ramaswamy, M Thirumal conceptualized this work and contributed to the writing.

Acknowledgments

The authors express their sincere gratitude to the SRM College of Pharmacy, SRM Institute of Science and Technology faculty for extending their support during the research work. We would also like to thank the Department of nanotechnology research center, SRMIST, for providing the results of the GC–MS analysis.

Financial & competing interests disclosure

The authors have no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties.

No writing assistance was utilized in the production of this manuscript.