Abstract
Introduction
Melasma is a condition of hyperpigmentation of the facial skin that increases in prevalence with ageing. The alleged involvement of reactive oxygen species and antioxidants is the basis of the pathology of melasma. Glutathione is one of the non-enzymatic antioxidants produced by the body and plays a role in melanogenesis. The purpose of this study was to examine serum glutathione levels on the severity of melasma.
Methods
This study used a cross-sectional design conducted at the Cosmetic Dermatology Clinic at Sanglah Hospital, Denpasar, from September to October 2016. Serum glutathione was examined through venous blood with ELISA method, and the severity of melasma was assessed using melasma area severity index (MASI). Independent t-test and ANOVA were used to evaluate differences in plasma glutathione levels based on the characteristics of the sample. Pearson correlation test and linear regression were used to assess the relationship between MASI and plasma glutathione.
Results
This study involved 47 people with a clinical diagnosis of melasma. There was a significant strong negative correlation between plasma glutathione and MASI (p<0.001; r = −0.624). Mild melasma (1.89 ± 0.28 µmol/L) had higher plasma glutathione levels compared to moderate melasma (1.53 ± 0.13 µmol/L) and severe (1.18 ± 0.20 µmol/L) (p=0.043). Linear regression showed a significant negative linear relationship between MASI scores against plasma glutathione (β = −58.2; p <0.01).
Conclusion
Glutathione plasma has a strong negative correlation with the MASI score in person with melasma.
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Ethical Aspect
All study protocols had been approved by the Ethical Committee Faculty of Medicine/Sanglah General Hospital, Bali-Indonesia with ethical clearance reference number 1874/UN.14.2/Litbang/2016. All patients had written signed informed consent prior to any data collection. All study procedure was conducted in accordance with the declaration of Helsinki.
Disclosure
The authors declare there are no conflicts of interest regarding the publication of the current study.