Chum salmon egg extracts induce upregulation of collagen type I and exert antioxidative effects on human dermal fibroblast cultures

Abstract

Components of fish roe possess antioxidant and antiaging activities, making them potentially very beneficial natural resources. Here, we investigated chum salmon eggs (CSEs) as a source of active ingredients, including vitamins, unsaturated fatty acids, and proteins. We incubated human dermal fibroblast cultures for 48 hours with high and low concentrations of CSE extracts and analyzed changes in gene expression. Cells treated with CSE extract showed concentration-dependent upregulation of collagen type I genes and of multiple antioxidative genes, including OXR1, TXNRD1, and PRDX family genes. We further conducted in silico phylogenetic footprinting analysis of promoter regions. These results suggested that transcription factors such as acute myeloid leukemia-1a and cyclic adenosine monophosphate response element-binding protein may be involved in the observed upregulation of antioxidative genes. Our results support the idea that CSEs are strong candidate sources of antioxidant materials and cosmeceutically effective ingredients.

Keywords:

• fish egg
• antiaging
• gene expression analysis
• antioxidative gene
• phylogenetic footprinting analysis

Supplementary material

Figure S1 Visualization of the results of promoter analysis of the PRDX4 gene, especially the CREB transcription factor-binding motif.

Notes: The upper left part of the figure shows the orthologous species alignment. The upper right panel shows the Pareto front graph with the CREB motif allocated on the first Pareto front, indicating its strong cross-species conservation and strong matching with a known matrix. The table below lists all candidate motifs with their similarity matching score to the consensus motif and their Pareto front number.

Abbreviation: CREB, cyclic adenosine monophosphate response element-binding protein.

Disclosure

The authors report no conflicts of interest in this work.